![cold as ice avicii sample cold as ice avicii sample](https://i.ytimg.com/vi/4kn6Fz2KNMw/maxresdefault.jpg)
![cold as ice avicii sample cold as ice avicii sample](https://i.ytimg.com/vi/D4rLAJ4g7e4/maxresdefault.jpg)
The following problems can arise if the reagents and samples are not kept cold throughout the preparation and partially degraded: If the PCR set up is done without adequate care, the result can be misleading, and the experiment a waste of time. Keeping the reagents cold during PCR set up will protect the samples and reagents intact without any degradation, and will produce the most sensitive and consistent results. Specially samples of RNA, and Taq DNA polymerase enzyme. It is important to keep the reagents and samples for PCR cold to avoid thermal degradation. The steps before PCR, the sample prep is critical to the success of PCR. For complex and sensitive samples any element of PCR, no matter how simple can affect the results with serious consequences, and costly waste of samples and reagents. How the samples are handled, how it is protected from degradation etc. For the complex specimens, careful preparation of the samples is critical to achieve best results.
![cold as ice avicii sample cold as ice avicii sample](http://d32ogoqmya1dw8.cloudfront.net/images/microbelife/IcecoreBC.jpg)
As the sample type gets complex, the technique also becomes complex, including sample prep for PCR. PCR has become a widely used tool in basic research as well as clinical diagnostics of a variety of infectious diseases.